Clinical Science Research Core

CSRC Director and Clinical Studies Performance Sub-core Director: Bettina Mittendorfer, PhD
Phone: 314-362-8450
Email: mittendb@wustl.edu

CSRC Metabolic Kinetics Sub-core Director: Bruce Patterson, PhD
Phone: 314-747-1575
Email: bpatters@wustl.edu

CSRC Metabolic Kinetics Sub-core Assistant Director: Gary Patti, PhD
Phone: 314-935-3512
Email: gjpattij@wustl.edu

Location

The CSRC provides services through dedicated office, clinical, and laboratory space in the Division of Geriatrics and Nutritonal Sciences located on the 2nd floor of the West Building and the 5th floor of the adjacent Irene Walter Johnson Building, and a number of shared resources within a few minutes walking distance on the Washington University School of Medicine (WUSM) campus, which makes it possible to leverage services and reduce cost.

Visit the Clinical Science Research Core website »


The goal of the CSRC is to support and enhance leading-edge clinical and translational nutrition/obesity research at Washington University School of Medicine (WUSM) by providing:

  • Comprehensive and affordable services that assist investigators in designing and implementing both simple and complex clinical and translational research protocols, obtaining reliable measures of key outcomes, and interpreting the results
  • Services that make it possible for young investigators, non-physician investigators, and basic scientists to conduct sophisticated clinical and translational research studies
  • Training and education in key areas of clinical and translational nutrition/obesity research for both young and established investigators and their research staff

To achieve these goals, we have developed two distinct but interactive sub-cores that provide the following specific services to investigators through a cohesive, “one-stop” organizational structure that ensures optimal integration of high-quality services.

The Clinical Studies Performance Sub-core (Director: Bettina Mittendorfer, PhD) assists investigators in conducting clinical nutrition/obesity protocols by:

  1. Providing consultation in designing a clinical research study and in interpreting the results of the data generated by core analyses
  2. Providing biostatistical support to determine adequate sample size and appropriate statistical analysis
  3. Measuring body composition and energy expenditure, iv) developing and preparing study protocol-specific meals for metabolic testing and take-out meals for diet intervention studies
  4. Preparing stable isotopically labeled metabolic tracer and hormone infusates to assess metabolic function
  5. Obtaining muscle and adipose tissue biopsy samples and inserting radial artery catheters for blood sampling
  6. Evaluating cardiovascular function, ix) performing exercise and physical performance testing
  7. Providing inpatient and outpatient sleep monitoring services
  8. Providing assistance in implementing lifestyle interventions (diet manipulation, weight loss/weight gain, exercise training)
  9. Providing training in specific procedures to aid investigators in the performance of clinical studies

The Metabolic Kinetics sub-core (Director: Bruce Patterson, PhD) offers services which include:

  1. Providing consultation in designing protocols to evaluate metabolic kinetics (e.g., the hyperinsulinemic-euglycemic clamp procedure and frequently sampled intravenous glucose tolerance test in conjunction with stable isotopically-labeled tracer infusions to evaluate whole-body and tissue-specific insulin action and β-cell function) and in interpreting the results of the data generated by core analyses
  2. Mass-spectrometry analysis of biospecimens to determine tracer enrichment in metabolic substrates and macromolecules
  3. Mathematical modeling of tracer and non-tracer data to assess metabolic kinetics, and iv) providing education and training in specific analyses

Equipment

The major equipment items available through the Clinical Studies Performance Sub-core includes equipment that is housed in the Clinical Translational Research Unit (CTRU), and that is jointly maintained and/or operated by the CTRU and the NORC Clinical Studies Performance Sub-core:

(1)   Lunar iDXA with CoreScan™ software for body composition and bone mineral density analysis in persons weighing up to 204 kg (450 pounds)

(4)   Parvo Medics True Max 2400 indirect calorimetry systems

(2)   Cycle ergometers (a Lode upright and a Cateye reclining bike)

(1)   Woodway treadmill

(1)   Biodex System 4 Pro dynamometer

(1)   Hoist multi-station exercise gym

(1)   Cadwell Industries, Sierra Summit electromyography and nerve conduction assessment system

(1)   Sonosite ultrasound system to assist with vascular access line placement

(1)   Philips Epiq 7 ultrasound system to assess cardiovascular function, tissue perfusion, and liver stiffness

(1)   SphygmoCor system to assess vascular compliance and arterial stiffness

(2)   EndoPat 2000 systems to evaluate endothelial function

(1)   Helena Labs Plateletworks system to assess platelet function

(2)   Compumedics Grael PSG/EEG with video monitoring for inpatient sleep assessment

(2)   Alice PDx portable sleep diagnostic system for outpatient sleep assessment

Also available are physical activity, Holter, and continuous glucose monitors, and smart scales.

All rooms in the CTRU are equipped with a YSI analyzer to measure glucose and lactate, and vital sign monitoring systems; also available are body scales and stadiometers, ECG equipment, and a cardiac emergency cart.

In addition, the CTRU provides equipment for blood and tissue sample processing, storage and shipping in dedicated laboratory space, including laminar flow hoods, analytical scales, centrifuges, refrigerators, and freezers that are connected to a remote monitoring and notification system.

Equipment housed in the NORC exercise facility (5th floor Irene Walter Johnson Building) includes:

(1)   Woodway Desmo-M treadmill and 1 Quinton Q55 treadmill

(1)   Cateye recumbent cycle ergometer

(1)   Parvo Medics TrueMax 2400 metabolic cart

(4)   Treadmills

(2)   Elliptical exercise ergometers

(6)   Rowing ergometers

(4)   Monark cycle ergometers

(4)   Reebok spin cycles

(5)   Schwinn Airdynes

(1)   Schwinn exerciser cycle

(3)   Hoist multi-station weight machines

(3)   Weight benches

(2)   Pull-up/multi gym towers

(1)   Powertech Hack squat machine

(1)   Smith machine

(1)   Eccentron

(2)   Foam rollers

(43) Dumbbells, ranging from 1lb-70lb

(1)   Aerobic step platform

(14) Risers for aerobic step

(3)   Weight medicine balls

(5)   Stability balls

(10) Sand ankle/wrist weights

(1)   Bosu balance trainer

(4)   Miniature cones for agility drills

(1)   Training hurdle

(1)   Cardiac emergency cart with defibrillator

The exercise facility is also outfitted with a 4-lane 90-meter indoor track.

Equipment housed in the pharmacy-grade infusate preparation facility in the CTRU includes:

(1)   IsoTech MicroSphere – ISO Class 5 Compounding Aseptic Isolator for the preparation of IV admixtures

(1)   BAXA volumetric automated repeater pump – for fluid transfers during compounding of sterile preparations

(1)   Centrifuge – for preparation of peptides during compounding of sterile preparations

(1)   Mettler Toledo MS303S Balance – for compounding sterile preparations

(1)   Ohaus Adventurer Pro Balance – for compounding sterile preparations

(1)   Portable heating water bath – for the preparation of CSP (compounded sterile preparations) at specific temperature

(1)   Endosafe PTS – portable device used for bacterial endotoxin testing of CSPs

(1)   Vortex Genie mixer –for the preparation of dilutions for endotoxin testing

(4)   Refrigerators to store CSPs

(3)   Incubators – for environmental monitoring and sterility testing procedures (all require incubation at different temperatures)

(5)   Digital data logger thermometers – used for temperature monitoring

(1)   Hot plate – used for the preparation of thioglycolate media with indicator used for sterility testing of final product

(1)   IV inspection light box – used for end preparation assessment for visible particulate contamination against white and black background

Equipment available in the CTRU metabolic kitchen:

(1) Vulcan commercial 10 plate electric range

(1) Commercial countertop convection steamer

(1) Hobart LXEH commercial under-counter dishwasher

(1) Norlake Walk-in Refrigerator/Freezer

(1) Traulsen G-series reach-in refrigerator

(4) Traulsen G-series reach-in freezers

Assorted small equipment for ingredient measurement, formula mixing, food preparation and packaging, including six analytical balances, microwave ovens, a digital sonifier, stir plates, laboratory glassware, a Robot Coupe food processor, a stand mixer, blenders, vacuum food sealer, heat sealer, and digital thermometers.

Equipment housed in the Central Laboratory for Clinical Studies (CLCS) includes:

(1)   Roche Diagnostics c501 chemistry analyzer

(1)   Roche Diagnostics e601 immunoanalyzer

(1)   Beckman Coulter UniCel DxH600 hematology analyzer

(1)   Siemens Immulite 1000 immunoanalyzer

(1)   Singulex Erenna immunoanalyzer (digital single molecule counting)

(1)   ICN Biomedical 10/600 Plus gamma counter

(1)   Titertek Plus gamma counter

(1)   Bio-Tek ELx 800 ELISA plate reader

(1)   Bio-Tek Powerwave 200 plate reader

(18) Alarmed and monitored -70° C freezers

Assorted small equipment for sample processing (vortexers, heating blocks, water baths, analytical balances, stir plates, microfuge, pH meter, etc.)

The major equipment items available in the NORC Metabolic Kinetics Sub-core include equipment housed and operated in the GC/MS module of the Metabolic Kinetics Sub-core:

(1)   Hewlett Packard (Agilent) 5977B GC/MS MSD system (EI/PCI/NCI) with autosampler

(1)   Hewlett Packard (Agilent) 5973N GC/MS MSD system (EI) with autosampler

(1)   Hewlett Packard (Agilent) 5973inert GC/MS MSD system (EI) with autosampler, headspace injector, and flame ionization detector

(1)   Beckman/Coulter Optima LE-80K ultracentrifuge with 3 rotors

(1)   Beckman GS-6KR refrigerated centrifuge

(2)   Thermo Fisher SC250EXP0 SpeedVac centrifugal concentrators

(1)   Beckman DU-520 spectrophotometer

(1)   BioTek Epoch 2 plate reader with spectrophotometric detector

(2)   -20°C freezers

(9)   -80°C freezers that are connected to a remote monitoring and notification system

Assorted small equipment for sample processing (vortexers, heating blocks, water baths, analytical balances, stir plates, microfuge, pH meter, etc.)

Equipment housed and operated in the LC/MS and NMR module of the Metabolic Kinetics Sub-core:

(1)   Agilent UHD swarm QTOF (6545) with autosampler

(1)   Agilent UHD extended resolution QTOF (6540) with autosampler

(1)   Agilent QTOF (6530) with autosampler

(1)   SCIEX TripleTOF 5600+ QTOF with autosampler

(1)   Thermo Q-Exactive Plus Orbitrap with autosampler

(2)   Thermo tribrid ID-X Obritrap with autosampler

(1)   Waters Synapt XS QTOF with ion mobility and autosampler

(1)   Agilent QqQ (6460) with autosampler

(1)   Thermo TSQ Altis with autosampler

(1)   Acquity UPLC I-Class PLUS LC system

(4)   Agilent 1290 UPLC LC system

(3)   Agilent 1260 HPLC LC system

(1)   Eksigent UHPLC LC system

(4)   Dionext UltiMate 300 UHPLC LC system

(2)   Bruker 500 MHz NMR spectrometers with 5-channel probes

(3)   Beckman GS-6KR refrigerated centrifuge

(2)   Thermo Fisher SC250EXP0 SpeedVac centrifugal concentrators

(1)   Cytation 7 BioTek fluorescence microscope and plate reader

(1)   Thermo Fisher Nanodrop UV-VIS Spectrophotometer

(1)   MP Bio Tissue Homogenizer

(1)   Li-Cor Odyssessy CLx Imaging System for Western blots

(1)   Agilent Seahorse XF Metabolic Flux Analyzer

(8)   Thermo Fisher 6.5 Cu ft incubators

(4)   Thermo Fisher 4 ft biosafety cabinets

(5)   -20°C freezers

(8)   -80°C freezers that are connected to a remote monitoring and notification system

Assorted small equipment for sample processing (vortexers, heating blocks, water baths, analytical balances, stir plates, microfuge, pH meter, microscopes, etc.)

Services available

Download CSRC Service Request form (pdf)


  1. Consultation for study design, protocol development, sample collection and storage, and interpreting results of the data generated by core analyses
  2. Clinical Studies Performance Sub-core services
    1. Body composition analyses (DEXA)
    2. Indirect calorimetry
    3. Research meals
    4. Intravenous infusate preparation
    5. Clinical laboratory tests
    6. Clinical procedures (muscle and adipose tissue collection, radial arterial catheterization, cardiovascular assessments)
    7. Sleep monitoring
    8. Exercise and physical performance assessments
    9. Lifestyle intervention
    10. Biostatistical support
  3. Metabolic Kinetics Sub-core services
    1. Sample processing and GC/MS analysis
    2. Mathematical modeling of metabolic data
    3. Dynamic metabolomics
  4. Training and education

If you have any questions regarding the CSRC’s cost structure, please feel free to contact Bettina Mittendorfer: mittendb@wustl.edu

Please acknowledge the Washington University NORC, Nutrition Obesity Research Center, in your publications by: “Supported by NIH grant P30 DK056341 (Nutrition Obesity Research Center).”